Sadguru Healthcare

Mispa Lume is a highly compact molecular diagnostics system to simultaneously perform 16 samples at faster TAT for real time detection of target genes using RT-LAMP reaction.

Mispa Lume - Top Ranked RT-LAMP Analyzer
Mispa Lume is a very small molecular diagnostics machine that can run 16 samples simultaneously at a quicker TAT for real-time target gene identification utilizing RT-LAMP reaction.

What is LAMP Technology:
LAMP is the short form for Loop-mediated isothermal amplification, a gene amplification assay for detecting the target gene. LAMP is faster than traditional PCR at analyzing genetic material. Reverse-transcription LAMP (RT-LAMP) amplifies RNA gene targets and has been used to find the COVID-19 virus. RT-LAMP with crude RNA samples can be used for quick testing at the point of care because it can use patient samples directly. It is crucial to select a diagnostic technique that is simpler and quicker than RT-PCR, depending on the circumstance. Both RT-LAMP and LAMP use four to 6 primers that recognize the target DNA. A strand-displacing DNA polymerase starts the process, and 2 of the primers form loop structures that make it easier for the next amplification round.

Specialties of an RT-LAMP Analyzer
RT-LAMP requires a constant temperature range from 60-72°C. InstaLume RT-LAMP analyzer is capable of maintaining a constant temperature and real-time fluorescence detection that enables real-time gene amplification monitoring. The InstaLume analyzer can run 1 to 16 samples at a time. The results can be obtained regarding Ct (Cycle threshold) values. One of the advantages of this technology is that the consumables require only 2-8*C, while RT PCR machine consumables are to be stored at -20*C during shelf life and transportation. Again, the analytical process is very simple, two steps, and any junior technician with little training can operate the system efficiently. Other technologies require well-trained technicians owing to the complexity of the testing process and technology.

Comparison of RT-LAMP with other technologies for the Detection of SARS-CoV-2
RT-LAMP, as discussed, is a simple, sensitive, quick, and cost-effective method that can find pathogens early. The reagents storage is between 2-8*C, and better performance is reported from established lab validations. InstaLume and SARS-Cov-2 are suitable reagents for very less TAT, and you get the results in 35 minutes post-sampling.

Mispa Lume gives accurate results with 100% specificity, 99.3% accuracy, and 98.7% sensitivity for Covid-19 confirmatory test. LAMP machine is available with affordable investment in smaller labs. Any smaller labs with less turnover also can use this machine with limited test numbers at a time.

Mispa MagenTa is an Automated Nucleic Acid extraction machine. The indigenously developed Agappe-Chitra Magna suits best for Mispa MagenTa. The run is performed in 96 deep well plates using an 8 strip magnetic head. Mispa MagenTa is a fully walk away system from Lyse, Bind, Wash, Dry to Elute steps. Finally, the elute is delivered in the elution tube.

Mispa MagenTa is a fully automated compact nucleic acid extraction machine based on magnetic beads technology for high throughput applications providing the highest yield.

Nucleic acid extraction, also called nucleic acid isolation or nucleic acid purification, is a common but important task in a molecular biology lab. In many genetic and genomic studies, the first step is to separate the genetic material from the rest of the sample. Preparing good samples makes it more likely that your experiment will work and give you the desired results.

The optimal Automated Nucleic Acid Extraction Machine, most appropriate for locally created Agappe-Chitra Magna testing, is the Mispa MagenTa. 96 deep well plates are used for the run, and an 8-strip magnetic head is used. Lyse, Bind, Wash, Dry, and Elute are all completely automated processes in the Mispa MagenTa system. The elute was finally delivered to the elution well.

• Ultra micron size magnetic beads in Agappe-Chitra Magna provide more surface area for binding nucleic acids
• Eliminates the need for centrifugation and produces longer fragments of RNA/DNA strands
• Magnetic separation method helps maintain intact longer fragments of the viral RNA
• Faster time to result in Amplification Step
• Magnetic separation can also be used in batch processes with a multitude of samples
• Relatively easy to execute in high-throughput automated RNA isolation applications
• Mispa MagenTa is able to purify up to 32 samples simultaneously
• All samples replaced in individual channels to avoid contamination

Pre-analytics has become the need of time to optimally manage the productive workflow and operation in a laboratory. Mispa Label is a smart & innovative solution for simplified pre-analytics. Ease of use, minimal human error, connectivity & best turnaround time in Mispa Label makes it a perfect pre-analytical solution.

Other Details:

• 7” TFT Touch Screen display matching the eye contrast ratio
• 6 tube racks with capacity of 120 tubes
• Throughput of 450 Tubes/Hr
• Automatic tube positioning & identification
• Thermal printer with 3 modes (primary, standby & STAT)
• Customizable category mapping for the tubes
• Auxiliary printer available for abnormal container label printing (Urine container, Petri Dish etc)
• Flexible machine design saves valuable space in the lab
• HIS/LIS with Bi-Directional connectivity
• USB, Ethernet, WIFI interface.